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cck 8  (Dojindo Labs)


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    Dojindo Labs cck 8
    Cck 8, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 59470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cck+8+assay+kit/Cell+Counting+Kit-8/pmc13085010-99-20-21
    Average 99 stars, based on 59470 article reviews
    cck 8 - by Bioz Stars, 2026-09
    99/100 stars

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    CCK-8 Assay:

    Article Title: Screening of Low-Tumorigenic MDCK Cells for Potential Influenza Vaccine Substrates and Transcriptomic Analyses
    Article Snippet: The expansion ratio determination follows the subcultivation recommendations for MDCK cells (CCL-34) provided by ATCC, which we quantified as the maximum sustainable dilution factor. .. The growth curve analysis using the CCK-8 assay kit (Dojindo, Kumamoto, Japan, CK04) was performed according to the manufacturer’s protocol based on the water-soluble tetrazolium salt WST-8, a method widely applied for cell proliferation assessment. ..

    Article Title: Aminated fullerene-based nanoplatform enables synergistic VEGFR2-targeted anti-angiogenesis and tumor immunotherapy
    Article Snippet: .. Cell viability was detected using a CCK-8 assay kit (DOJINDO, Kumamoto, Japan). .. The absorbance at 450 nm was measured using a microplate reader (iMark microplate reader, Bio-RAD, USA) to determine cell viability (cell viability = (ODtre - ODmedium)/(ODcon - ODmedium)), where ODtre is the absorbance of treated cells at 450 nm, ODcon is the absorbance of control cells, and ODmedium is the absorbance of the culture medium.

    Article Title: LNGFR promoting osteogenic differentiation of ectomesenchyme stem cells via activation of GHR-JAK-STAT/IGF1 signaling pathway.
    Article Snippet: The migration area rate was calculated using the formula: Migration area rate (%) = [(A0 - A12) / A0] × 100%, where A0 denotes the initial scratch width and A12 represents the remaining scratch width at 12 h. All quantitative analyses were performed using ImageJ software (version 1.80, National Institutes of Health, USA). .. 2.8 CCK8 assay Cell proliferation of EMSCs was evaluated using a CCK-8 assay kit (Dojindo, Kumamoto, Japan). ..

    Article Title: Integrated cryopreservation-thawing-transplantation platform for neural stem cell-based spinal cord injury repair
    Article Snippet: .. NSCs were treated with composite materials at various time points and assessed for viability using the CCK-8 assay kit (Dojindo, Japan). ..

    Article Title: Multifunctional fiber-optic theranostic probe for closed-loop tumor photothermal therapy
    Article Snippet: Fetal bovine serum (FBS) was purchased from Shanghai ExCell Bio, Inc. (Shanghai, China). .. The CCK-8 assay kit was purchased from Dojindo Molecular Technologies, Inc. (Shanghai, China). ..

    Article Title: Polyphenol-Rich Extract from Archidendron clypearia : Optimization, Characterization, and Hypouricemic Activity.
    Article Snippet: .. CCK-8 assay kit was provided by Dojindo (Kumamoto, Japan). .. Phosphate-Buffered Saline was purchased from Procell (Wuhan, China).

    Article Title: Strontium and gallium doping enhances in vivo bone regeneration in biomimetic hydroxyapatite 3D-printed scaffolds
    Article Snippet: .. Cells were exposed to the extracts for 24 h, after which cell viability was assessed using a CCK-8 assay kit (Dojindo, Japan). .. The OD values were measured at 450 nm and the background absorbance at 650 nm was subtracted using a multimodal plate reader (Tecan).

    Article Title: Adipose‐Derived Stem Cells From Human Lumbar Subcutaneous Tissue in Obesity: Inflammatory Microenvironment Promotes Differentiation Capacity
    Article Snippet: One set of Passage 1 (P1) cells from Group N was continuously stimulated by supplementing the basal culture medium with 50 ng/mL IFN‐γ (R&D Systems, USA) and 10 ng/mL TNF‐α (R&D Systems, USA) and was designated as the Normol‐prestimulation group (Group N‐pre). .. The proliferative capacity of ADSCs was assessed using a CCK‐8 assay kit (Dojindo, Japan). ..



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    In vitro cytocompatibility assessment . <t>(a)</t> <t>CCK-8</t> assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    In vitro cytocompatibility assessment . <t>(a)</t> <t>CCK-8</t> assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    In vitro cytocompatibility assessment . <t>(a)</t> <t>CCK-8</t> assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    cck 8 assay kit  (Dojindo Labs)
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    In vitro cytocompatibility assessment . <t>(a)</t> <t>CCK-8</t> assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation

    doi: 10.1016/j.bioactmat.2026.04.003

    Figure Lengend Snippet: In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: On days 1, 3, and 5 of culture, the existing culture medium was substituted with 100 μL of CCK-8 working solution (Cell Counting Kit-8, Beyotime Biotech, China), prepared by mixing fresh α-MEM and CCK-8 reagent at a volume ratio of 10:1.

    Techniques: In Vitro, CCK-8 Assay, Cell Culture, Staining, Migration